For simple perfusion cultures of free-floating organoids or tissue spheroids, the organoids can be directly seeded into the middle wells without a gel. The dimensions of the inlet and outlet channels are adjustable for specific applications. Narrower channels (A001) are ideal for physically connect- ing to self-assembled vasculature or trapping organoids and spheroids in the middle wells in the absence of a gel. Wider channels (A001-2) are recommended for AngioTEER measurements of tissue barriers. Bi-directional interstitial flow in the gel matrix is achieved with the IFlowRocker, removing the need for pumps. The open-well design allows easy access for pipetting and tissue removal, facilitating down- stream analysis. The optically clear plate bottom supports high-content imaging with confocal microscopy and high-throughput analysis with plate readers. The 384-well design minimizes the use of cells and reagents, significantly reducing experimental costs per data point. To learn more, visit www.organo-biotech.com/posters.
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